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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [5]
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- Product number
- PA5-20337 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDA5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Daudi cell lysate.
- Concentration
- 1 mg/mL
Submitted references The Causal Role of Mitochondrial Dynamics in Regulating Innate Immunity in Diabetes.
Adenosine deaminase acting on RNA 1 limits RIG-I RNA detection and suppresses IFN production responding to viral and endogenous RNAs.
Chang YH, Lin HY, Shen FC, Su YJ, Chuang JH, Lin TK, Liou CW, Lin CY, Weng SW, Wang PW
Frontiers in endocrinology 2020;11:445
Frontiers in endocrinology 2020;11:445
Adenosine deaminase acting on RNA 1 limits RIG-I RNA detection and suppresses IFN production responding to viral and endogenous RNAs.
Yang S, Deng P, Zhu Z, Zhu J, Wang G, Zhang L, Chen AF, Wang T, Sarkar SN, Billiar TR, Wang Q
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3436-45
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3436-45
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Daudi cell lysate using a MDA5 polyclonal antibody (Product # PA5-20337) at (A) 1, (B) 2 and (C) 4 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MDA5 in Daudi cell lysate with MDA5 Polyclonal Antibody (Product # PA5-20337) at (A) 1, (B) 2 and (C) 4 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human lymph node cells using a MDA5 polyclonal antibody (Product # PA5-20337) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 Polyclonal Antibody (Product # PA5-20337) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 Polyclonal Antibody (Product # PA5-20337) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 Polyclonal Antibody (Product # PA5-20337) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Role of MFN1/MFN2 overexpression on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were overexpressed with GFP, GFP-MFN1, or GFP-MFN2 for 18 h, followed by a 6-h exposure with or without 0.25 mM FA. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Role of MFN1/MFN2 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. Quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA or between indicated groups. + p < 0.05 comparison between indicated groups. CON, control; FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Role of DRP1/FIS1 knockdown on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to siRNA control in the absence of FA. + p < 0.05 or between indicated groups. CON, control; FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Role of DRP1/FIS1 overexpression on innate immune response in cybrid B4 under nutrient excess. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to vector control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effect of mdivi-1 on innate immune response in cybrid B4 under nutrient excess. Cybrid B4 cells were treated with/without FA for 6 h, followed by 10 muM mdivi-1 for another 24 h. (A) Representative blot image of RIG-I, MDA5, MAVS, NLRP3, and caspase-1. beta-Actin as loading control. (B) Histograms of densitometric results. The quantification value of beta-actin was used for normalization. * p < 0.05 compared to mock control in the absence of FA. + p < 0.05 comparison between indicated groups. FA, fatty acid.
