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Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX60368 - Provider product page
- Provider
- GeneTex
- Product name
- RFXAP antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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- Experimental details
- ChIP assays were performed using NALM cells, RFX-AP antibody and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 £gg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).
