AP6503b

antibody from Abgent

Targeting: IL1RN ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6503b

Provider

Abgent

Proper citation

Abgent Cat#AP6503b, RRID:AB_1967390

Product name

IL1RN Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"IL1RN Antibody (C-term) (Cat.# AP6503b) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the IL1RN antibody detected the IL1RN protein (arrow)."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of IL1RN Antibody (C-term)(Cat#AP6503b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

IL1RN Antibody (C-term) (Cat. #AP6503b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50