11090-1-AP

antibody from Invitrogen Antibodies

Targeting: DNAJC7 TPR2, TTC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

11090-1-AP

Provider

Invitrogen Antibodies

Product name

DNAJC7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: YEKIAETDF EKRDFRKVVF CMDRALEFAP ACHRFKILKA ECLAMLGRYP EAQSVASDIL RMDSTNADAL YVRGLCLYYE DCIEKAVQFF VQALRMAPDH EKACIACRNA KALKAKKEDG NKAFKEGNYK LAYELYTEAL GIDPNNIKTN AKLYCNRGTV NSKLRKLDDA IEDCTNAVKL DDTYIKAYLR RAQCYMDTEQ YEEAVRDYEK VYQTEKTKEH KQLLKNAQLE LKKSKRKDYY KILGVDKNAS EDEIKKAYRK RALMHHPDRH SGASAEVQKE EEKKFKEVGE AFTILSDPKK KTRYDSGQDL DEEGMNMGDF DPNNIFKAFF GGPGGFSFEA SGPGNFFFQF G (145-494 aa encoded by BC011837)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using DNAJC7 antibody 11090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11090-1-AP (DNAJC7 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11090-1-AP (DNAJC7 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug DNAJC7 antibody (11090-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.