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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX121474 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121474, RRID:AB_10723235
- Product name
- ISG15 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE GTX121474 diluted at 1:2000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 £gg) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ISG15 antibody detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde for 10 min.Green: ISG15 protein stained by ISG15 antibody (GTX121474) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ISG15 antibody(GTX121474) antibody at 1:500 dilution.
