A-830

antibody from R&D Systems

Targeting: ISG15 G1P2, IFI15, UCRP

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

A-830

Provider

R&D Systems

Product name

Human ISG15/UCRP Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. This antibody will recover ISGylated proteins in immunoprecipitation applications. This antibody detects ISGylated proteins in Western blots.

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P05161


Isotype

IgG

Antibody clone number

851701

Vial size

50 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.

References

Submitted references

Covalent ISG15 conjugation to CHIP promotes its ubiquitin E3 ligase activity and inhibits lung cancer cell growth in response to type I interferon.

Yoo L, Yoon AR, Yun CO, Chung KC
Cell death & disease 2018 Jan 24;9(2):97

Immunoprecipitation

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Immunoprecipitation A549 cells transfected with HA-ISG15 were treated briefly with IFN gamma or carrier only. 500 µg of cell lysates were subjected to immunoprecipitation (IP) with 20 µg A-830 using Protein G Sepharose. IP’d material was resolved on reducing SDS-PAGE gels and Western Blots were developed using PVDF membranes and anti-HA epitope (R&D Systems MAB060) at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Free (unconjugated) HA-ISG15 was visualized in both treated and untreated cells, while ISGylated proteins were clearly recovered (IP’d) from the interferon-treated cell lysates.