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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1720 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1720, RRID:AB_1238084
- Product name
- ISG15 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ISG15.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Interferon stimulated gene 15 constitutively produced by melanoma cells induces e-cadherin expression on human dendritic cells.
IFN-stimulated gene 15 is synergistically activated through interactions between the myelocyte/lymphocyte-specific transcription factors, PU.1, IFN regulatory factor-8/IFN consensus sequence binding protein, and IFN regulatory factor-4: characterization of a new subtype of IFN-stimulated response element.
Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element.
Padovan E, Terracciano L, Certa U, Jacobs B, Reschner A, Bolli M, Spagnoli GC, Borden EC, Heberer M
Cancer research 2002 Jun 15;62(12):3453-8
Cancer research 2002 Jun 15;62(12):3453-8
IFN-stimulated gene 15 is synergistically activated through interactions between the myelocyte/lymphocyte-specific transcription factors, PU.1, IFN regulatory factor-8/IFN consensus sequence binding protein, and IFN regulatory factor-4: characterization of a new subtype of IFN-stimulated response element.
Meraro D, Gleit-Kielmanowicz M, Hauser H, Levi BZ
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6224-31
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6224-31
Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element.
Reich N, Evans B, Levy D, Fahey D, Knight E Jr, Darnell JE Jr
Proceedings of the National Academy of Sciences of the United States of America 1987 Sep;84(18):6394-8
Proceedings of the National Academy of Sciences of the United States of America 1987 Sep;84(18):6394-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The ISG15 polyclonal antibody (Cat # PAB1720) is used in Western blot to detect ISG15 in mouse kidney tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ISG15 polyclonal antibody (Cat # PAB1720) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
