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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB4845 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ISG15/UCRP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ISG15/UCRP in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
ABW03539- Isotype
- IgG
- Antibody clone number
- 539442
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Vitamin D receptor and Jak-STAT signaling crosstalk results in calcitriol-mediated increase of hepatocellular response to IFN-α.
Lange CM, Gouttenoire J, Duong FH, Morikawa K, Heim MH, Moradpour D
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):6037-44
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):6037-44
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human ISG15/UCRP by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
