PA5-22969

antibody from Invitrogen Antibodies

Targeting: GPX3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22969

Provider

Invitrogen Antibodies

Product name

GPX3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The target sequence has 89% sequence homology with mouse, 88% sequence homology with bovine and 84% sequence homology with porcine.

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GPX3 in human kidney lysate. Sample was incubated in GPX3 polyclonal antibody (Product # PA5-22969).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GPX3 using a polyclonal antibody (Product # PA5-22969).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of GPX3 in formalin fixed paraffin-embedded (FFPE) human kidney. Samples were incubated in GPX3 polyclonal antibody (Product # PA5-22969) using a dilution of 1:200. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Glutathione Peroxidase 3 was observed. Staining was performed by Histowiz.