PAB29357

antibody from Abnova Corporation

Targeting: CYC1 UQCR4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29357

Provider

Abnova Corporation

Product name

CYC1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human CYC1.

Antigen sequence

NSEAARAANNGALPPDLSYIVRARHGGEDYVFSLL
TGYCEPPTGVSLREGLYFNPYFPGQAIAMAPPIYT
DVLEFDDGTPATMSQIAKDVCTFLRWASEPEHDHR
KRMGLKMLMMMALLVPLVYTIKRHKWSVLKSRKLA
YR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with CYC1 polyclonal antibody (Cat # PAB29357) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with CYC1 polyclonal antibody (Cat # PAB29357) at 1-4 ug/mL concentration shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with CYC1 polyclonal antibody (Cat # PAB29357) shows strong cytoplasmic positivity in parietal cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)