Antibody data
- Antibody Data
- Antigen structure
- References [45]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 15440-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15440-1-AP, RRID:AB_2137152
- Product name
- LONP1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated LONP1 antibody (Cat. #15440-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.
UPRmt activation protects against MPP+-induced toxicity in a cell culture model of Parkinson's disease.
Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer's disease-like pathology in human cerebral organoids.
The parkinsonian LRRK2 R1441G mutation shows macroautophagy-mitophagy dysregulation concomitant with endoplasmic reticulum stress.
DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion.
Self-assembly of multi-component mitochondrial nucleoids via phase separation.
Calpain-Inhibitors Protect Frataxin-Deficient Dorsal Root Ganglia Neurons from Loss of Mitochondrial Na+/Ca2+ Exchanger, NCLX, and Apoptosis.
An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.
Calcitriol increases frataxin levels and restores mitochondrial function in cell models of Friedreich Ataxia.
LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.
SIRT3-mediated mitochondrial unfolded protein response weakens breast cancer sensitivity to cisplatin.
Mitochondrial Lon protease is a gatekeeper for proteins newly imported into the matrix.
ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice.
Identification of a Novel c-Myc Inhibitor 7594-0037 by Structure-Based Virtual Screening and Investigation of Its Anti-Cancer Effect on Multiple Myeloma.
Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.
miR-29a Modulates GSK3β/SIRT1-Linked Mitochondrial Proteostatic Stress to Ameliorate Mouse Non-Alcoholic Steatohepatitis.
A High-Density Human Mitochondrial Proximity Interaction Network.
ATF4 Mediates Mitochondrial Unfolded Protein Response in Alveolar Epithelial Cells.
Exogenous Therapeutics of Microrna-29a Attenuates Development of Hepatic Fibrosis in Cholestatic Animal Model through Regulation of Phosphoinositide 3-Kinase p85 Alpha.
Toxicity of Necrostatin-1 in Parkinson's Disease Models.
Loss of high-temperature requirement protein A2 protease activity induces mitonuclear imbalance via differential regulation of mitochondrial biogenesis in sarcopenia.
Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.
Glucose-regulated protein 75 in foodborne disease models induces renal tubular necrosis.
TRPC6-Mediated ERK1/2 Activation Increases Dentate Granule Cell Resistance to Status Epilepticus Via Regulating Lon Protease-1 Expression and Mitochondrial Dynamics.
TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.
Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.
Mutant huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability.
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.
CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.
Impaired Mitophagy and Protein Acetylation Levels in Fibroblasts from Parkinson's Disease Patients.
Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
Hispidulin alleviates high-glucose-induced podocyte injury by regulating protective autophagy.
Zinc enhances the cellular energy supply to improve cell motility and restore impaired energetic metabolism in a toxic environment induced by OTA.
Lon protease: a novel mitochondrial matrix protein in the interconnection between drug-induced mitochondrial dysfunction and endoplasmic reticulum stress.
Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
miR-542-3p suppresses invasion and metastasis by targeting the proto-oncogene serine/threonine protein kinase, PIM1, in melanoma.
A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.
Circadian Oscillation of Sulfiredoxin in the Mitochondria.
RNA sequencing identifies dysregulation of the human pancreatic islet transcriptome by the saturated fatty acid palmitate.
Central role of Nix in the autophagic response to ochratoxin A.
The transcription factor C/EBP delta has anti-apoptotic and anti-inflammatory roles in pancreatic beta cells.
Decreased activity of the 20S proteasome in the brain white matter and gray matter of patients with multiple sclerosis.
Sun D, Niu Z, Zheng HX, Wu F, Jiang L, Han TQ, Wei Y, Wang J, Jin L
Cellular and molecular gastroenterology and hepatology 2021;11(4):1211-1226.e15
Cellular and molecular gastroenterology and hepatology 2021;11(4):1211-1226.e15
UPRmt activation protects against MPP+-induced toxicity in a cell culture model of Parkinson's disease.
Hu D, Liu Z, Qi X
Biochemical and biophysical research communications 2021 Sep 10;569:17-22
Biochemical and biophysical research communications 2021 Sep 10;569:17-22
Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer's disease-like pathology in human cerebral organoids.
Pérez MJ, Ivanyuk D, Panagiotakopoulou V, Di Napoli G, Kalb S, Brunetti D, Al-Shaana R, Kaeser SA, Fraschka SA, Jucker M, Zeviani M, Viscomi C, Deleidi M
Molecular psychiatry 2021 Oct;26(10):5733-5750
Molecular psychiatry 2021 Oct;26(10):5733-5750
The parkinsonian LRRK2 R1441G mutation shows macroautophagy-mitophagy dysregulation concomitant with endoplasmic reticulum stress.
Yakhine-Diop SMS, Rodríguez-Arribas M, Canales-Cortés S, Martínez-Chacón G, Uribe-Carretero E, Blanco-Benítez M, Duque-González G, Paredes-Barquero M, Alegre-Cortés E, Climent V, Aiastui A, López de Munain A, Bravo-San Pedro JM, Niso-Santano M, Fuentes JM, González-Polo RA
Cell biology and toxicology 2021 May 31;
Cell biology and toxicology 2021 May 31;
DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion.
Silva-Pinheiro P, Pardo-Hernández C, Reyes A, Tilokani L, Mishra A, Cerutti R, Li S, Rozsivalova DH, Valenzuela S, Dogan SA, Peter B, Fernández-Silva P, Trifunovic A, Prudent J, Minczuk M, Bindoff L, Macao B, Zeviani M, Falkenberg M, Viscomi C
Nucleic acids research 2021 May 21;49(9):5230-5248
Nucleic acids research 2021 May 21;49(9):5230-5248
Self-assembly of multi-component mitochondrial nucleoids via phase separation.
Feric M, Demarest TG, Tian J, Croteau DL, Bohr VA, Misteli T
The EMBO journal 2021 Mar 15;40(6):e107165
The EMBO journal 2021 Mar 15;40(6):e107165
Calpain-Inhibitors Protect Frataxin-Deficient Dorsal Root Ganglia Neurons from Loss of Mitochondrial Na+/Ca2+ Exchanger, NCLX, and Apoptosis.
Britti E, Delaspre F, Tamarit J, Ros J
Neurochemical research 2021 Jan;46(1):108-119
Neurochemical research 2021 Jan;46(1):108-119
An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.
Jin X, Zhang Z, Nie Z, Wang C, Meng F, Yi Q, Chen M, Sun J, Zou J, Jiang P, Guan MX
The Journal of biological chemistry 2021 Jan-Jun;296:100437
The Journal of biological chemistry 2021 Jan-Jun;296:100437
Calcitriol increases frataxin levels and restores mitochondrial function in cell models of Friedreich Ataxia.
Britti E, Delaspre F, Sanz-Alcázar A, Medina-Carbonero M, Llovera M, Purroy R, Mincheva-Tasheva S, Tamarit J, Ros J
The Biochemical journal 2021 Jan 15;478(1):1-20
The Biochemical journal 2021 Jan 15;478(1):1-20
LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.
Shin CS, Meng S, Garbis SD, Moradian A, Taylor RW, Sweredoski MJ, Lomenick B, Chan DC
Nature communications 2021 Jan 11;12(1):265
Nature communications 2021 Jan 11;12(1):265
SIRT3-mediated mitochondrial unfolded protein response weakens breast cancer sensitivity to cisplatin.
Chen H, Zhang DM, Zhang ZP, Li MZ, Wu HF
Genes & genomics 2021 Dec;43(12):1433-1444
Genes & genomics 2021 Dec;43(12):1433-1444
Mitochondrial Lon protease is a gatekeeper for proteins newly imported into the matrix.
Matsushima Y, Takahashi K, Yue S, Fujiyoshi Y, Yoshioka H, Aihara M, Setoyama D, Uchiumi T, Fukuchi S, Kang D
Communications biology 2021 Aug 16;4(1):974
Communications biology 2021 Aug 16;4(1):974
ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
Shu L, Hu C, Xu M, Yu J, He H, Lin J, Sha H, Lu B, Engelender S, Guan M, Song Z
The EMBO journal 2021 Apr 15;40(8):e106283
The EMBO journal 2021 Apr 15;40(8):e106283
Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice.
Zhou Q, Zhu L, Qiu W, Liu Y, Yang F, Chen W, Xu R
International journal of biological sciences 2020;16(2):284-297
International journal of biological sciences 2020;16(2):284-297
Identification of a Novel c-Myc Inhibitor 7594-0037 by Structure-Based Virtual Screening and Investigation of Its Anti-Cancer Effect on Multiple Myeloma.
Yao R, Xie Y, Sun X, Zhang M, Zhou J, Liu L, Gao J, Xu K
Drug design, development and therapy 2020;14:3983-3993
Drug design, development and therapy 2020;14:3983-3993
Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.
Sun D, Yao S, Wu F, Deng W, Ma Y, Jin L, Wang J, Wang X
Frontiers in genetics 2020;11:577795
Frontiers in genetics 2020;11:577795
miR-29a Modulates GSK3β/SIRT1-Linked Mitochondrial Proteostatic Stress to Ameliorate Mouse Non-Alcoholic Steatohepatitis.
Yang YL, Wang PW, Wang FS, Lin HY, Huang YH
International journal of molecular sciences 2020 Sep 19;21(18)
International journal of molecular sciences 2020 Sep 19;21(18)
A High-Density Human Mitochondrial Proximity Interaction Network.
Antonicka H, Lin ZY, Janer A, Aaltonen MJ, Weraarpachai W, Gingras AC, Shoubridge EA
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Cell metabolism 2020 Sep 1;32(3):479-497.e9
ATF4 Mediates Mitochondrial Unfolded Protein Response in Alveolar Epithelial Cells.
Jiang D, Cui H, Xie N, Banerjee S, Liu RM, Dai H, Thannickal VJ, Liu G
American journal of respiratory cell and molecular biology 2020 Oct;63(4):478-489
American journal of respiratory cell and molecular biology 2020 Oct;63(4):478-489
Exogenous Therapeutics of Microrna-29a Attenuates Development of Hepatic Fibrosis in Cholestatic Animal Model through Regulation of Phosphoinositide 3-Kinase p85 Alpha.
Yang YL, Wang FS, Lin HY, Huang YH
International journal of molecular sciences 2020 May 21;21(10)
International journal of molecular sciences 2020 May 21;21(10)
Toxicity of Necrostatin-1 in Parkinson's Disease Models.
Alegre-Cortés E, Muriel-González A, Canales-Cortés S, Uribe-Carretero E, Martínez-Chacón G, Aiastui A, López de Munain A, Niso-Santano M, Gonzalez-Polo RA, Fuentes JM, Yakhine-Diop SMS
Antioxidants (Basel, Switzerland) 2020 Jun 15;9(6)
Antioxidants (Basel, Switzerland) 2020 Jun 15;9(6)
Loss of high-temperature requirement protein A2 protease activity induces mitonuclear imbalance via differential regulation of mitochondrial biogenesis in sarcopenia.
Zhou H, Yuan D, Gao W, Tian J, Sun H, Yu S, Wang J, Sun L
IUBMB life 2020 Aug;72(8):1659-1679
IUBMB life 2020 Aug;72(8):1659-1679
Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.
Shen Y, Ding M, Xie Z, Liu X, Yang H, Jin S, Xu S, Zhu Z, Wang Y, Wang D, Xu L, Zhou X, Wang P, Bi J
Frontiers in cellular neuroscience 2019;13:568
Frontiers in cellular neuroscience 2019;13:568
Glucose-regulated protein 75 in foodborne disease models induces renal tubular necrosis.
Yang X, Li Y, Zheng L, He X, Luo Y, Huang K, Xu W
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2019 Nov;133:110720
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2019 Nov;133:110720
TRPC6-Mediated ERK1/2 Activation Increases Dentate Granule Cell Resistance to Status Epilepticus Via Regulating Lon Protease-1 Expression and Mitochondrial Dynamics.
Kim JE, Park H, Choi SH, Kong MJ, Kang TC
Cells 2019 Nov 1;8(11)
Cells 2019 Nov 1;8(11)
TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.
Wang P, Deng J, Dong J, Liu J, Bigio EH, Mesulam M, Wang T, Sun L, Wang L, Lee AY, McGee WA, Chen X, Fushimi K, Zhu L, Wu JY
PLoS genetics 2019 May;15(5):e1007947
PLoS genetics 2019 May;15(5):e1007947
Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.
Hu D, Sun X, Liao X, Zhang X, Zarabi S, Schimmer A, Hong Y, Ford C, Luo Y, Qi X
Acta neuropathologica 2019 Jun;137(6):939-960
Acta neuropathologica 2019 Jun;137(6):939-960
Mutant huntingtin inhibits the mitochondrial unfolded protein response by impairing ABCB10 mRNA stability.
Fu Z, Liu F, Liu C, Jin B, Jiang Y, Tang M, Qi X, Guo X
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1428-1435
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1428-1435
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Rudler DL, Hughes LA, Perks KL, Richman TR, Kuznetsova I, Ermer JA, Abudulai LN, Shearwood AJ, Viola HM, Hool LC, Siira SJ, Rackham O, Filipovska A
Science advances 2019 Dec;5(12):eaay2118
Science advances 2019 Dec;5(12):eaay2118
Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.
Ferreira N, Perks KL, Rossetti G, Rudler DL, Hughes LA, Ermer JA, Scott LH, Kuznetsova I, Richman TR, Narayana VK, Abudulai LN, Shearwood AJ, Cserne Szappanos H, Tull D, Yeoh GC, Hool LC, Filipovska A, Rackham O
The EMBO journal 2019 Dec 16;38(24):e102155
The EMBO journal 2019 Dec 16;38(24):e102155
CDDO-Me Selectively Attenuates CA1 Neuronal Death Induced by Status Epilepticus via Facilitating Mitochondrial Fission Independent of LONP1.
Kim JE, Park H, Choi SH, Kong MJ, Kang TC
Cells 2019 Aug 5;8(8)
Cells 2019 Aug 5;8(8)
Impaired Mitophagy and Protein Acetylation Levels in Fibroblasts from Parkinson's Disease Patients.
Yakhine-Diop SMS, Niso-Santano M, Rodríguez-Arribas M, Gómez-Sánchez R, Martínez-Chacón G, Uribe-Carretero E, Navarro-García JA, Ruiz-Hurtado G, Aiastui A, Cooper JM, López de Munaín A, Bravo-San Pedro JM, González-Polo RA, Fuentes JM
Molecular neurobiology 2019 Apr;56(4):2466-2481
Molecular neurobiology 2019 Apr;56(4):2466-2481
Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels.
Iida R, Ueki M, Yasuda T
Oxidative medicine and cellular longevity 2018;2018:6956414
Oxidative medicine and cellular longevity 2018;2018:6956414
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.
Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005
Cell reports 2018 Jun 5;23(10):2989-3005
Hispidulin alleviates high-glucose-induced podocyte injury by regulating protective autophagy.
Wu F, Li S, Zhang N, Huang W, Li X, Wang M, Bai D, Han B
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Aug;104:307-314
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Aug;104:307-314
Zinc enhances the cellular energy supply to improve cell motility and restore impaired energetic metabolism in a toxic environment induced by OTA.
Yang X, Wang H, Huang C, He X, Xu W, Luo Y, Huang K
Scientific reports 2017 Nov 7;7(1):14669
Scientific reports 2017 Nov 7;7(1):14669
Lon protease: a novel mitochondrial matrix protein in the interconnection between drug-induced mitochondrial dysfunction and endoplasmic reticulum stress.
Polo M, Alegre F, Moragrega AB, Gibellini L, Marti-Rodrigo A, Blas-Garcia A, Esplugues JV, Apostolova N
British journal of pharmacology 2017 Dec;174(23):4409-4429
British journal of pharmacology 2017 Dec;174(23):4409-4429
Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
Di K, Lomeli N, Wood SD, Vanderwal CD, Bota DA
Oncotarget 2016 Nov 22;7(47):77457-77467
Oncotarget 2016 Nov 22;7(47):77457-77467
miR-542-3p suppresses invasion and metastasis by targeting the proto-oncogene serine/threonine protein kinase, PIM1, in melanoma.
Rang Z, Yang G, Wang YW, Cui F
Biochemical and biophysical research communications 2016 May 27;474(2):315-320
Biochemical and biophysical research communications 2016 May 27;474(2):315-320
A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.
Pryde KR, Taanman JW, Schapira AH
Cell reports 2016 Dec 6;17(10):2522-2531
Cell reports 2016 Dec 6;17(10):2522-2531
Circadian Oscillation of Sulfiredoxin in the Mitochondria.
Kil IS, Ryu KW, Lee SK, Kim JY, Chu SY, Kim JH, Park S, Rhee SG
Molecular cell 2015 Aug 20;59(4):651-63
Molecular cell 2015 Aug 20;59(4):651-63
RNA sequencing identifies dysregulation of the human pancreatic islet transcriptome by the saturated fatty acid palmitate.
Cnop M, Abdulkarim B, Bottu G, Cunha DA, Igoillo-Esteve M, Masini M, Turatsinze JV, Griebel T, Villate O, Santin I, Bugliani M, Ladriere L, Marselli L, McCarthy MI, Marchetti P, Sammeth M, Eizirik DL
Diabetes 2014 Jun;63(6):1978-93
Diabetes 2014 Jun;63(6):1978-93
Central role of Nix in the autophagic response to ochratoxin A.
Shen XL, Zhang B, Liang R, Cheng WH, Xu W, Luo Y, Zhao C, Huang K
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Jul;69:202-9
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Jul;69:202-9
The transcription factor C/EBP delta has anti-apoptotic and anti-inflammatory roles in pancreatic beta cells.
Moore F, Santin I, Nogueira TC, Gurzov EN, Marselli L, Marchetti P, Eizirik DL
PloS one 2012;7(2):e31062
PloS one 2012;7(2):e31062
Decreased activity of the 20S proteasome in the brain white matter and gray matter of patients with multiple sclerosis.
Zheng J, Bizzozero OA
Journal of neurochemistry 2011 Apr;117(1):143-53
Journal of neurochemistry 2011 Apr;117(1):143-53
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15440-1-AP(LONP1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The LONP1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human LONP1. This antibody recognizes human,mouse,rat antigen. The LONP1 antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-LONP1 (IP:15440-1-AP, 3ug; Detection:15440-1-AP 1:1000) with mouse heart tissue lysate 9500ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 15440-1-AP(LONP1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 15440-1-AP(LONP1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue