16004-1-AP

antibody from Invitrogen Antibodies

Targeting: NBR1 1A1-3B, CA125, KIAA0049, M17S2

Provider product page for 16004-1-AP

Western blot

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [8]
Immunocytochemistry [1]
Immunohistochemistry [8]
Flow cytometry [1]
Other assay [1]

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Product number: 16004-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: NBR1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: MEPQVTLNV TFKNEIQSFL VSDPENTTWA DIEAMVKVSF DLNTIQIKYL DEENEEVSIN SQGEYEEALK MAVKQGNQLQ MQVHEGHHVV DEAPPPVVGA KRLAARAGKK PLAHYSSLVR VLGSDMKTPE DPAVQSFPLV PCDTDQPQDK PPDWFTSYLE TFREQVVNET VEKLEQKLHE KLVLQNPSLG SCPSEVSMPT SEETLFLPEN QFSWHIACNN CQRRIVGVRY QCSLCPSYNI CEDCEAGPYG HDTNHVLLKL RRPVVGSSEP FCHSKYSTPR LPAALEQVRL QKQVDKNFLK AEKQRLRAEK KQRKAEVKEL KKQLKLHRKI HLWNSIHGLQ SPKSPLGRPE SLLQ (1-353 aa encoded by BC009808)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.42 mg/mL
Storage: -20°C

NBR1 protein structure - 16004-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A2780 cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: COS-7 cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer using 16004-1-AP (NBR1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer using 16004-1-AP (NBR1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 16004-1-AP (NBR1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 16004-1-AP (NBR1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).