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- References [7]
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- Product number
- NBP1-71703 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71703, RRID:AB_11003500
- Product name
- Rabbit Polyclonal NBR1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.24 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Depletion of NBR1 in urothelial carcinoma cells enhances rapamycin-induced apoptosis through impaired autophagy and mitochondrial dysfunction.
Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains.
Clinical isolates of the modern Mycobacterium tuberculosis lineage 4 evade host defense in human macrophages through eluding IL-1β-induced autophagy.
NBR1 enables autophagy-dependent focal adhesion turnover.
Loss of Atg12, but not Atg5, in pro-opiomelanocortin neurons exacerbates diet-induced obesity.
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
Recessive mutations in EPG5 cause Vici syndrome, a multisystem disorder with defective autophagy.
Kim MJ, Hwang GY, Cho MJ, Chi BH, Park SI, Chang IH, Whang YM
Journal of cellular biochemistry 2019 Nov;120(11):19186-19201
Journal of cellular biochemistry 2019 Nov;120(11):19186-19201
Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains.
Di Rienzo M, Antonioli M, Fusco C, Liu Y, Mari M, Orhon I, Refolo G, Germani F, Corazzari M, Romagnoli A, Ciccosanti F, Mandriani B, Pellico MT, De La Torre R, Ding H, Dentice M, Neri M, Ferlini A, Reggiori F, Kulesz-Martin M, Piacentini M, Merla G, Fimia GM
Science advances 2019 May;5(5):eaau8857
Science advances 2019 May;5(5):eaau8857
Clinical isolates of the modern Mycobacterium tuberculosis lineage 4 evade host defense in human macrophages through eluding IL-1β-induced autophagy.
Romagnoli A, Petruccioli E, Palucci I, Camassa S, Carata E, Petrone L, Mariano S, Sali M, Dini L, Girardi E, Delogu G, Goletti D, Fimia GM
Cell death & disease 2018 May 24;9(6):624
Cell death & disease 2018 May 24;9(6):624
NBR1 enables autophagy-dependent focal adhesion turnover.
Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, Wittmann T, Debnath J
The Journal of cell biology 2016 Feb 29;212(5):577-90
The Journal of cell biology 2016 Feb 29;212(5):577-90
Loss of Atg12, but not Atg5, in pro-opiomelanocortin neurons exacerbates diet-induced obesity.
Malhotra R, Warne JP, Salas E, Xu AW, Debnath J
Autophagy 2015;11(1):145-54
Autophagy 2015;11(1):145-54
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function.
Murrow L, Malhotra R, Debnath J
Nature cell biology 2015 Mar;17(3):300-10
Nature cell biology 2015 Mar;17(3):300-10
Recessive mutations in EPG5 cause Vici syndrome, a multisystem disorder with defective autophagy.
Cullup T, Kho AL, Dionisi-Vici C, Brandmeier B, Smith F, Urry Z, Simpson MA, Yau S, Bertini E, McClelland V, Al-Owain M, Koelker S, Koerner C, Hoffmann GF, Wijburg FA, ten Hoedt AE, Rogers RC, Manchester D, Miyata R, Hayashi M, Said E, Soler D, Kroisel PM, Windpassinger C, Filloux FM, Al-Kaabi S, Hertecant J, Del Campo M, Buk S, Bodi I, Goebel HH, Sewry CA, Abbs S, Mohammed S, Josifova D, Gautel M, Jungbluth H
Nature genetics 2013 Jan;45(1):83-7
Nature genetics 2013 Jan;45(1):83-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.
