CABT-L627

antibody from Creative Diagnostics

Targeting: SGCD CMD1L, DAGD, LGMD2F

Western blot (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

CABT-L627

Provider

Creative Diagnostics

Product name

Rabbit Anti-CDC7 Kinase monoclonal antibody, clone TZ32-12

Antibody type

Monoclonal

Description

The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7/Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase a-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4. The kinase activity of the complex is regulated throughout the cell cycle, mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

TZ32-12

Vial size

100 _l

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.