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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- TA319508 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Ajuba antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Ajuba antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- AJUBA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.67 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head). ?Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.? Detection occurred using a 1:10,000 dilution of IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Ajuba antibody shows detection of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead). ?Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates.IP of Ajuba followed by WB may result in cleaner background staining.Primary antibody was used at 1:500.? Detection occurred using a 1:5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG.
- Validation comment
- WB
