PAB9930

AJUBA antibody from Abnova Corporation

JUB, MGC15563

Western blot

Supportive data in Antibodypedia

Provider product page for PAB9930

Antibody Data

Product number

PAB9930

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9930, RRID:AB_1676555

Product name

JUB polyclonal antibody

Provider product page

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of JUB.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
Hirota T, Kunitoku N, Sasayama T, Marumoto T, Zhang D, Nitta M, Hatakeyama K, Saya H
Cell 2003 Sep 5;114(5):585-98

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The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin.
Marie H, Pratt SJ, Betson M, Epple H, Kittler JT, Meek L, Moss SJ, Troyanovsky S, Attwell D, Longmore GD, Braga VM
The Journal of biological chemistry 2003 Jan 10;278(2):1220-8

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Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner.
Goyal RK, Lin P, Kanungo J, Payne AS, Muslin AJ, Longmore GD
Molecular and cellular biology 1999 Jun;19(6):4379-89

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of a 57 KDa band consistent with the expected MW for JUB (arrowhead).Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A-431, 4) Jurkat and 5) 293 whole cell lysates.Immunoprecipitation of JUB followed by western blotting may result in cleaner background staining.Approximately 5 ug ofeach preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of JUB polyclonal antibody.Detection occurred using a 1 : 5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.Achemiluminescence system was used for signal detection using a 60-sec exposure time.Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of JUB-RFP fusion protein in cell lysates (arrow-head).Lanes correspond to 1) vector only transfection, 2) human JUB-RFP, 3) mouse JUB-RFP, and 4) mock transfection.Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1 : 1,700 dilution of JUB polyclonal antibody.Detection occurred using a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nmchannel, green).Molecular weight estimation wasmade by comparison to prestained MW markers (indicated at left, 700 nm channel, red).IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.IRDye is a trademark of LI-COR, Inc.