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LearnAP51231PU-N
antibody from Acris Antibodies GmbH
Targeting: DEFB1
BD1, DEFB-1, DEFB101, HBD-1, HBD1, MGC51822
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51231PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51231PU-N, RRID:AB_11144100
- Product name
- anti Defensin beta 1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of Human DEFB1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the DEFB1 antibody detected the DEFB1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N with MDA-MB231 cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human kidney tissue stained with Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB231 cells using Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
