ABIN951877
antibody from antibodies-online
Targeting: DEFB1
BD1, DEFB-1, DEFB101, HBD-1, HBD1, MGC51822
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN951877 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Defensin, beta 1 (DEFB1) (N-Term), (AA 11-37) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 11-37 amino acids from the N-terminal region of Human DEFB1. Genename: DEFB1
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 11-37
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the DEFB1 antibody detected the DEFB1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N with MDA-MB231 cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human kidney tissue stained with Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB231 cells using Defensin beta 1 Antibody (N-term) Cat.-No AP51231PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.