A303-821A

antibody from Invitrogen Antibodies

Targeting: PSMC1 p56, S4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-821A

Provider

Invitrogen Antibodies

Product name

PSMC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PSMC1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from 293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody A303-821A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PSMC1 was not successfully immunoprecipitated by rabbit anti-PSMC1 antibody A303-820A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse PSMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 (Cat. No. A303-821A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PSMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 (Cat. No. A303-821A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.