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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- AF8198 - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha-Defensin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human alpha-Defensin 1 in direct ELISAs.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P59665- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human alpha-Defensin 1 ELISA Standard Curve. Recombinant Human alpha-Defensin 1 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha-Defensin 1 Monoclonal Antibody (Catalog # MAB8198) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human alpha-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- alpha-Defensin 1 in Human Small Intestine. alpha-Defensin 1 was detected in formalin fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human alpha-Defensin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8198) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to lamina propria in intestinal villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
