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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA328734 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-TRPC4
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-TRPC4
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Trpc4
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes: 1. Anti-TRPC4 antibody, (1:200). 2. Anti-TRPC4 antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PC3 (lanes 1,3) and LNCaP cell lysates: 1. Anti-TRPC4 antibody, (1:200). 2. Anti-TRPC4 antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of rat brain lysate: 1. Rat brain lysate. 2. Lysate immunoprecipitated with Anti-TRPC4 antibody, (6 mg). 3. Lysate immunoprecipitated with pre-immune rabbit serum. The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain. Western blot analysis was performed with Anti-TRPC4 antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPC4 in mouse cerebellum. Immunohistochemical staining of mouse cerebellum frozen sections with Anti-TRPC4 antibody. A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
- Validation comment
- IHC
