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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF1036 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-MSK1 (S212) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human MSK1 when phosphorylated at S212.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells.
Tamgüney T, Zhang C, Fiedler D, Shokat K, Stokoe D
Experimental cell research 2008 Jul 1;314(11-12):2299-312
Experimental cell research 2008 Jul 1;314(11-12):2299-312
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-MSK1 (S212) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM H2O2 for 1 hour or 300 mM sorbitol for 30 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-MSK1 (S212) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1036), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSK1 (S212) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MSK1 in Human Kidney Cancer Tissue. MSK1 phosphorylated at S212 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Rabbit Anti-Human Phospho-MSK1 (S212) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1036) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
