Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2518 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse MSK1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse MSK1 in Western blots. This antibody does not detect recombinant human MSK2.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O75582- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CALHM1 controls the Ca²⁺-dependent MEK, ERK, RSK and MSK signaling cascade in neurons.
Glucocorticoid receptors recruit the CaMKIIα-BDNF-CREB pathways to mediate memory consolidation.
Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning.
Dreses-Werringloer U, Vingtdeux V, Zhao H, Chandakkar P, Davies P, Marambaud P
Journal of cell science 2013 Mar 1;126(Pt 5):1199-206
Journal of cell science 2013 Mar 1;126(Pt 5):1199-206
Glucocorticoid receptors recruit the CaMKIIα-BDNF-CREB pathways to mediate memory consolidation.
Chen DY, Bambah-Mukku D, Pollonini G, Alberini CM
Nature neuroscience 2012 Dec;15(12):1707-14
Nature neuroscience 2012 Dec;15(12):1707-14
Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning.
Sindreu CB, Scheiner ZS, Storm DR
Neuron 2007 Jan 4;53(1):79-89
Neuron 2007 Jan 4;53(1):79-89
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse MSK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK1 was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MSK1 in HeLa Human Cell Line. MSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
