Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
Reference
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- Product number
- AB0112-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0112-200, RRID:AB_2333145
- Product name
- Anti-HIF1a
- Antibody type
- Polyclonal
- Description
- Huntingtin is a protein linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance.
Insulin resistance is associated with tissue-specific regulation of HIF-1α and HIF-2α during mild chronic intermittent hypoxia.
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.
Rodrigues T, Matafome P, Sereno J, Almeida J, Castelhano J, Gamas L, Neves C, Gonçalves S, Carvalho C, Arslanagic A, Wilcken E, Fonseca R, Simões I, Conde SV, Castelo-Branco M, Seiça R
Scientific reports 2017 May 10;7(1):1698
Scientific reports 2017 May 10;7(1):1698
Insulin resistance is associated with tissue-specific regulation of HIF-1α and HIF-2α during mild chronic intermittent hypoxia.
Sacramento JF, Ribeiro MJ, Rodrigues T, Guarino MP, Diogo LN, Seiça R, Monteiro EC, Matafome P, Conde SV
Respiratory physiology & neurobiology 2016 Jul;228:30-8
Respiratory physiology & neurobiology 2016 Jul;228:30-8
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.
Ferreira JV, Soares AR, Ramalho JS, Pereira P, Girao H
Scientific reports 2015 May 11;5:10210
Scientific reports 2015 May 11;5:10210
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Anti-HIF1a Ab (AB0112) at 1/1,000 dilution; MBP-HIF1a C-term recombinant protein and 293 cells transfected with myc-his-HIF1a in the presence of CoCl2; lysate at 100 µg per lane
- Sample type
- MBP-HIF1a C-term protein and 293HEK cells transfected with myc-his-HIF1a
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- HT1080 cells in the presence of CoCl2; lysate at 100 µg per lane;
- Sample type
- HT1080 cells
- Primary Ab dilution
- 1/2,500
- Conjugate
- Unconjugated
- Secondary Ab dilution
- 1/10,000