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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AB0070-100 - Provider product page
- Provider
- SICGEN
- Product name
- Anti-HIF1a
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to insulin receptor. INSR is a receptor tyrosine kinase. Preprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. The insulin signalling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein is activated by the binding of insulin or other ligands to this receptor.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 300 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia.
Ramalho AR, Toscano A, Pereira P, Girão H, Gonçalves L, Marques C
Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology 2017 May;36(5):367-373
Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology 2017 May;36(5):367-373
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Supportive validation
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- SICGEN (provider)
- Main image
- Experimental details
- HT1080 cells stimulated with CoCl2; lysate at 100 µg per lane
- Sample type
- HT1080 cell line
- Primary Ab dilution
- 1:2,500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:10,000
