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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- ARG65509 - Provider product page
- Provider
- Arigo
- Product name
- anti-Notch1 antibody [mN1A]
- Antibody type
- Monoclonal
- Antigen
- GST fusion protein containing cdc10-NCR region of mouse Notch1
- Description
- Purified from cell culture supernatant by protein-A affinity chromatography.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- mN1A
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at 2-8°C. Do not freeze. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 1) Jurkat or 2) mouse thymocytes cell lysate were stained with anti-Notch 1 [mN1A] antibody. The clone mN1A recognizes both mouse and human full-length Notch1 (270 kD) and cleaved Notch1 (110-120 kD).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: (A) CHO and (B) MCF-7 cells were stained with Notch1 antibody [mN1A] (black) and Isotype control (gray). Antibody usage: 1 µg antibody per 1 x 10^6 cells.
