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- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [15]
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- Product number
- TA500078 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500078, RRID:AB_2282907
- Product name
- Anti-Notch1 mouse monoclonal antibody, clone OTI3E12 (formerly 3E12)
- Antibody type
- Monoclonal
- Description
- Anti-Notch1 mouse monoclonal antibody, clone OTI3E12 (formerly 3E12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NOTCH1
- Isotype
- IgG
- Antibody clone number
- OTI3E12
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
Submitted references Notch1-Hes1 signalling axis in the tumourigenesis of biliary neuroendocrine tumours.
Harada K, Sato Y, Ikeda H, Hsu M, Igarashi S, Nakanuma Y
Journal of clinical pathology 2013 May;66(5):386-91
Journal of clinical pathology 2013 May;66(5):386-91
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1(RC211365) (RC211365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-Notch1 mouse monoclonal antibody (TA500078) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1(RC211365)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-Notch1 mouse monoclonal antibody (TA500078).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-Notch1 mouse monoclonal antibody (TA500078).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-Notch1(TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas within the normal limits using anti-Notch1(TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-Notch1(TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1(TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry for Notch1: Comparative analysis of Notch1 expression between human adenocarcinomas and neuroendocrine tumours (NETs) by using anti-Notch 1 antibody. Nuclear expression is found in the adenocarcinomas (A and B, arrows), but rarely in NETs (A and B, *). Original magnification, (A), ?40; (B), ?200. Dilution: 1:40
- Validation comment
- IHC
