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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-36015 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF599 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: SK-N-SH, SK-N-SH nuclear extract.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZNF599 was performed by separating 30 µg of SK-N-SH whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF599 Polyclonal Antibody (Product # PA5-36015) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF599 Polyclonal Antibody detects ZNF599 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF599 protein stained by ZNF599 Polyclonal Antibody (Product # PA5-36015) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ZNF599 was performed in paraffin-embedded human breast carcinoma tissue using ZNF599 Polyclonal Antibody (Product # PA5-36015) at a dilution of 1:500.
