PAB28624

antibody from Abnova Corporation

Targeting: CTSS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28624

Provider

Abnova Corporation

Product name

CTSS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CTSS.

Antigen sequence

NGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQY
DSKYRAATCSKYTELPYGREDVLKEAVANKGPVSV
GVDARHPSFFLYRSGVYYEPSCTQNVNHGVLVVGY
GDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHC
G


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with CTSS polyclonal antibody (Cat # PAB28624) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CTSS polyclonal antibody (Cat # PAB28624) at 1-4 ug/ml shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human spleen with CTSS polyclonal antibody (Cat # PAB28624) shows strong cytoplasmic positivity in cells in red pulp at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)