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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-47088 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cathepsin S Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
- Concentration
- 0.2 mg/mL
Submitted references Cathepsin S Cleaves BAX as a Novel and Therapeutically Important Regulatory Mechanism for Apoptosis.
Soond SM, Savvateeva LV, Makarov VA, Gorokhovets NV, Townsend PA, Zamyatnin AA Jr
Pharmaceutics 2021 Mar 5;13(3)
Pharmaceutics 2021 Mar 5;13(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KD of Cathepsin S was achieved by transfecting A549 with Cathepsin S specific siRNAs (Silencer® select Product # s3762, s3763). Western blot analysis (Fig. a) was performed using whole cell extracts from the Cathepsin S KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Cathepsin S Polyclonal Antibody (Product # PA5-47088, 1µg/mL) and Rabbit Anti-Goat IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27014,0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram where Fig. b corresponds to KD of 37 kDa band and Fig. c corresponds to that of 24kDa). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Cathepsin S..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cathepsin S Polyclonal Antibody (Product # PA5-47088) and 37 kDa, 24 kDa bands corresponding to Cathepsin S was observed upon treatments in A549 cells. Whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with IFN gamma (50 ng/mL for 12hr) (Lane 2) and A549 treated with IFN gamma (50 ng/mL for 24hr) (Lane 3) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence Rabbit Anti-Goat IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Cathepsin S in immersion fixed paraffin-embedded sections of human lymph node. Samples were incubated in Cathepsin S polyclonal antibody (Product # PA5-47088) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
