Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AF1183 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cathepsin S Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P25774
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity.
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer.
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors.
Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases.
Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation.
Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis.
Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity.
Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
A multiplex immunoassay for human adipokine profiling.
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Upregulation of cathepsin S in the aging and pathological nervous system of mice.
Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Bailleul G, Kravtzoff A, Joulin-Giet A, Lecaille F, Labas V, Meudal H, Loth K, Teixeira-Gomes AP, Gilbert FB, Coquet L, Jouenne T, Brömme D, Schouler C, Landon C, Lalmanach G, Lalmanach AC
PloS one 2016;11(8):e0161573
PloS one 2016;11(8):e0161573
Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer.
Liu WL, Liu D, Cheng K, Liu YJ, Xing S, Chi PD, Liu XH, Xue N, Lai YZ, Guo L, Zhang G
Oncotarget 2016 May 10;7(19):28124-38
Oncotarget 2016 May 10;7(19):28124-38
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors.
Huang CC, Lee CC, Lin HH, Chang JY
Scientific reports 2016 Jul 8;6:29256
Scientific reports 2016 Jul 8;6:29256
Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases.
Hamon Y, Legowska M, Hervé V, Dallet-Choisy S, Marchand-Adam S, Vanderlynden L, Demonte M, Williams R, Scott CJ, Si-Tahar M, Heuzé-Vourc'h N, Lalmanach G, Jenne DE, Lesner A, Gauthier F, Korkmaz B
The Journal of biological chemistry 2016 Apr 15;291(16):8486-99
The Journal of biological chemistry 2016 Apr 15;291(16):8486-99
Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
Brindle NR, Joyce JA, Rostker F, Lawlor ER, Swigart-Brown L, Evan G, Hanahan D, Shchors K
PloS one 2015;10(4):e0120348
PloS one 2015;10(4):e0120348
Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation.
Barry ZT, Platt MO
The Journal of biological chemistry 2012 Aug 10;287(33):27723-30
The Journal of biological chemistry 2012 Aug 10;287(33):27723-30
Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis.
Naudin C, Joulin-Giet A, Couetdic G, Plésiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G
PloS one 2011;6(9):e25577
PloS one 2011;6(9):e25577
Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity.
Kwok HF, Buick RJ, Kuehn D, Gormley JA, Doherty D, Jaquin TJ, McClurg A, Ward C, Byrne T, Jaworski J, Leung KL, Snoddy P, McAnally C, Burden RE, Gray B, Lowry J, Sermadiras I, Gruszka N, Courtenay-Luck N, Kissenpfennig A, Scott CJ, Johnston JA, Olwill SA
Molecular cancer 2011 Dec 14;10:147
Molecular cancer 2011 Dec 14;10:147
Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
Shree T, Olson OC, Elie BT, Kester JC, Garfall AL, Simpson K, Bell-McGuinn KM, Zabor EC, Brogi E, Joyce JA
Genes & development 2011 Dec 1;25(23):2465-79
Genes & development 2011 Dec 1;25(23):2465-79
A multiplex immunoassay for human adipokine profiling.
Schipper HS, de Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clinical chemistry 2010 Aug;56(8):1320-8
Clinical chemistry 2010 Aug;56(8):1320-8
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Wendt W, Schulten R, Stichel CC, Lübbert H
Journal of neurochemistry 2009 Sep;110(6):1931-41
Journal of neurochemistry 2009 Sep;110(6):1931-41
Upregulation of cathepsin S in the aging and pathological nervous system of mice.
Wendt W, Lübbert H, Stichel CC
Brain research 2008 Sep 26;1232:7-20
Brain research 2008 Sep 26;1232:7-20
Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
Genes & development 2006 Mar 1;20(5):543-56
Genes & development 2006 Mar 1;20(5):543-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cathepsin S in Human Lymph Node. Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.