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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- PA1-28109 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Concentration
- 1.0 mg/mL
Submitted references TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy.
Zheng N, Xie K, Ye H, Dong Y, Wang B, Luo N, Fan J, Tan J, Chen W, Yu X
JCI insight 2020 Jul 23;5(14)
JCI insight 2020 Jul 23;5(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR7 in TLR7 using a TLR7 polyclonal antibody (Product # PA1-28109) at a dilution of 3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TLR7 was performed by loading RAW264.7 cell lysate. Proteins were transferred to a membrane and probed with a TLR7 Polyclonal Antibody (Product # PA1-28109).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TLR7 in Ramos cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and TLR7 Polyclonal Antibody (Product # PA1-28109) (Green) at a dilution of 1:50 was used as a primary antibody. Red : Tubulin. Blue: DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TLR7 in RAW264.7 cells using TLR7 Polyclonal Antibody (Product # PA1-28109) (Green) at a dilution of 1:10. Red : Tubulin. Blue: DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TLR7 in human colon tissue using TLR7 Polyclonal Antibody (Product # PA1-28109) at a dilution of 5 µg/mL. TOP : primary antibodyBottom : isotype control
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PEX19 was performed in mouse splenocytes using PEX19 Monoclonal Antibody (GT533) (Product # MA5-17281) (Red). Green : isotype control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis analysis of human peripheral blood mononuclear cell (PBMC) using TLR7 Polyclonal Antibody (Product # PA1-28109). Red: Primary antibody. Green: isotype control. Shaded histogram: cell only. Dilution: 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of ATP6IP2 was performed in human monocyte using a ATP6IP2 Polyclonal Antibody (Product # PA1-32079) (Red) at a dilution of 0.5 µg/10^6 cells. Green : isotype control. Shaded histogram : cells only.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of GADD34 was performed in human peripheral blood mononuclear cells (PBMCs) using GADD34 Polyclonal Antibody (Product # PA1-33115) (Red). Green : isotype control.
