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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- IC5875P - Provider product page
- Provider
- R&D Systems
- Product name
- Human TLR7 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR7 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q9NYK1- Isotype
- IgG
- Antibody clone number
- 533707
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Peripheral blood and hepatic Toll-like receptor 7 expression and interferon lambda 1 levels in chronic hepatitis C: Relation to virus replication and liver injury.
Expression of Toll-like receptor -7 and -9 in B cell subsets from patients with primary Sjögren's syndrome.
Global analyses revealed age-related alterations in innate immune responses after stimulation of pathogen recognition receptors.
A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
PD-1 negatively regulates interleukin-12 expression by limiting STAT-1 phosphorylation in monocytes/macrophages during chronic hepatitis C virus infection.
El Aggan H, Farahat N, El Deeb N, Zeid A, El-Shendidi A
Microbial pathogenesis 2019 Jun;131:65-74
Microbial pathogenesis 2019 Jun;131:65-74
Expression of Toll-like receptor -7 and -9 in B cell subsets from patients with primary Sjögren's syndrome.
Karlsen M, Hansen T, Nordal HH, Brun JG, Jonsson R, Appel S
PloS one 2015;10(3):e0120383
PloS one 2015;10(3):e0120383
Global analyses revealed age-related alterations in innate immune responses after stimulation of pathogen recognition receptors.
Metcalf TU, Cubas RA, Ghneim K, Cartwright MJ, Grevenynghe JV, Richner JM, Olagnier DP, Wilkinson PA, Cameron MJ, Park BS, Hiscott JB, Diamond MS, Wertheimer AM, Nikolich-Zugich J, Haddad EK
Aging cell 2015 Jun;14(3):421-32
Aging cell 2015 Jun;14(3):421-32
A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
Swaminathan G, Rossi F, Sierra LJ, Gupta A, Navas-Martín S, Martín-García J
PLoS pathogens 2012 Sep;8(9):e1002937
PLoS pathogens 2012 Sep;8(9):e1002937
PD-1 negatively regulates interleukin-12 expression by limiting STAT-1 phosphorylation in monocytes/macrophages during chronic hepatitis C virus infection.
Ma CJ, Ni L, Zhang Y, Zhang CL, Wu XY, Atia AN, Thayer P, Moorman JP, Yao ZQ
Immunology 2011 Mar;132(3):421-31
Immunology 2011 Mar;132(3):421-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PE-conjugated Monoclonal Antibody (Catalog # IC5875P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
