AF-219-NA

antibody from Novus Biologicals

Targeting: IL12A CLMF, IL-12A, NFSK, NKSF1, p35

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-219-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-12 Antibody

Antibody type

Polyclonal

References

Submitted references

Mycobacterium bovis Bacillus Calmette-Guérin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.

Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
International immunology 2008 Oct;20(10):1321-9

---

Upregulated IL-23 and IL-17 in Behçet patients with active uveitis.

Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
Investigative ophthalmology & visual science 2008 Jul;49(7):3058-64

---

Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.

Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
European journal of immunology 2008 Apr;38(4):1012-23

---

Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.

Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5284-92

---

Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.

Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5284-92

---

Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes.

Koelle DM, Huang J, Hensel MT, McClurkan CL
Journal of virology 2006 Mar;80(6):2863-72

---

Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.

Nagarajan S, Selvaraj P
Vaccine 2006 Mar 20;24(13):2264-74

---

Effect of piceatannol in human monocyte-derived dendritic cells in vitro.

Takei M, Umeyama A, Arihara S, Matsumoto H
Journal of pharmaceutical sciences 2005 May;94(5):974-82

---

Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.

Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86

---

Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.

Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86

---

Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.

Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5213-21

---

Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.

Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5213-21

---

APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.

Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):138-43

---

APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.

Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):138-43

---

NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.

Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7

---

NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.

Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
Journal of immunology (Baltimore, Md. : 1950) 2004 Jan 1;172(1):130-7

---

Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.

Artavanis-Tsakonas K, Eleme K, McQueen KL, Cheng NW, Parham P, Davis DM, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5396-405

---

Activation of a subset of human NK cells upon contact with Plasmodium falciparum-infected erythrocytes.

Artavanis-Tsakonas K, Eleme K, McQueen KL, Cheng NW, Parham P, Davis DM, Riley EM
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5396-405

---

Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.

Hessian PA, Highton J, Kean A, Sun CK, Chin M
Arthritis and rheumatism 2003 Feb;48(2):334-8

---

Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma.

Nutku E, Gounni AS, Olivenstein R, Hamid Q
The Journal of allergy and clinical immunology 2000 Aug;106(2):288-92

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-12 and Neutralization by Human IL-12 Antibody. Recombinant Human IL-12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 0.03-0.05 µg/mL.