Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21598 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2L3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, HepG2, Neuro2A, GL261, C8D30, mouse testis, whole zebrafish.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UBE2L3 was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a UBE2L3 Polyclonal Antibody (Product # PA5-21598) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE2L3 Polyclonal Antibody (Product # PA5-21598). Sample (30 µg of whole cell lysate). Lane A: Hela . Lane B: Hep G2. 12% SDS PAGE. UBE2L3 Polyclonal Antibody (Product # PA5-21598) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UBE2L3 Polyclonal Antibody (Product # PA5-21598). Whole zebrafish extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 Polyclonal Antibody (Product # PA5-21598) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UBE2L3 Polyclonal Antibody detects UBE2L3 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.15 % SDS-PAGE. UBE2L3 Polyclonal Antibody (Product # PA5-21598) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ube2L3 in paraformaldehyde-fixed HeLa cells using a Ube2L3 polyclonal antibody (Product # PA5-21598) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3 (Product # PA5-21598) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3 (Product # PA5-21598) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.