NBP1-30995

antibody from Novus Biologicals

Targeting: UBE2L3 UBCH7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-30995

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-30995, RRID:AB_2304029

Product name

Rabbit Polyclonal UbcH7/UBE2L3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified.

Reactivity

Human, Mouse, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract, C. 30 ug C8D30 whole cell lysate/extract 15 % SDS-PAGE gel, antibody dilution 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody (GTX104717).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody (GTX104717).

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: UbcH7/UBE2L3 Antibody [NBP1-30995] - Immunoprecipitation of UbcH7/UBE2L3 protein from HeLa whole cell extracts using 5 ug of UbcH7/UBE2L3 antibody (NBP1-30995). Western blot analysis was performed using UbcH7/UBE2L3 antibody (NBP1-30995). EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP1-30995] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.