H00000002-D01

antibody from Abnova Corporation

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000002-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000002-D01, RRID:AB_10549730

Product name

A2M MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human A2M protein.

Antigen sequence

MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLV
PSLLHTETTEKGCVLLSYLNETVTVSASLESVRGN
RSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTV
QVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKP
GQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRI
AQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKK
SGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEE
MNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHG
EDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKE
YEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKL
SFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIF
IRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLT
VRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPS
KSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGL
KKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFS
ISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDV
ENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVC
ALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGF
PGPLNDQDDEDCINRHNVYINGITYTPVSSTNEKD
MYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGP
EGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYF
PETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAF
CLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRG
EAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVE
KEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAE
ALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGL
EKETTFNSLLCPSGGEVSEELSLKLPPNVVEESAR
ASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLF
APNIYVLDYLNETQQLTPEVKSKAIGYLNTGYQRQ
LNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFA
QARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGS
LLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPV
VRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFA
LAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPK
APVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTS
EDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHA
LSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNN
NRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKY
NILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISL
SVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLE
RSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQ
DVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKD
LGNA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

A2M MaxPab rabbit polyclonal antibody. Western Blot analysis of A2M expression in human placenta.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of A2M expression in transfected 293T cell line (H00000002-T01) by A2M MaxPab polyclonal antibody.Lane 1: A2M transfected lysate(163.30 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of A2M transfected lysate using anti-A2M MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with A2M purified MaxPab mouse polyclonal antibody (B01P) (H00000002-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol