PB9853

antibody from Boster Biological Technology

Targeting: CP

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PB9853

Provider

Boster Biological Technology

Product name

Anti-Ceruloplasmin/CP Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CERULOPLASMIN/Cp detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. CERULOPLASMIN/Cp information: Molecular Weight: 121151 MW; Subcellular Localization: Secreted. Colocalizes with GCP1 in secretory intracellular compartments; Tissue Specificity: Expressed in many tissues, including liver, eye and brain.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Ceruloplasmin/CP using anti- Ceruloplasmin/CP antibody (PB9853). Ceruloplasmin/CP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Ceruloplasmin/CP Antibody (PB9853) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image