Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA1-29882 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PEDF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Recommended positive controls: RIPA lysates from Y79 cells. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Y79 cell lysate using PEDF Polyclonal Antibody (Product # PA1-29882). Dilution: 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PEDF Polyclonal Antibody (Product # PA1-29882) and a 46k Da band corresponding to PEDF was observed in Hep G2 treated with protein transport inhibitor but not in SK-OV-3 upon same treatment. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with 1X protein transport inhibitor for 4 hours (Lane 2), SK-OV-3 (Lane 3) or SK-OV-3 treated with 1X protein transport inhibitor for 4 hours (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A non-specific band observed close to 150 kDa has been marked with an asterisk.