Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00000183-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000183-M04, RRID:AB_1237638
- Product name
- AGT monoclonal antibody (M04), clone 3C10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant AGT.
- Antigen sequence
MRKRAPQSEMAPAGVSLRATILCLLAWAGLAAGDR
VYIHPFHLVIHNESTCEQLAKANAGKPKDPTFIPA
PIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAM
VGMLANFLGFRIYGMHSELWGVVHGATVLSPTAVF
GTLASLYLGALDHTADRLQAILGVPWKDKNCTSRL
DAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVG
VFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELD
VAAEKIDRFMQAVTGWKTGCSLTGASVDSTLAFNT
YVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSG
MGTFQHWSDIQDNFSVTQVSFTESACLLLIQPHYA
SDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLV
LQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIR
VGEVLNSIFFELEADEREPTESTQQLNKPEVLEVT
LNRPFLFAVYDQSATALHFLGRVANPLSTA- Isotype
- IgG
- Antibody clone number
- 3C10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of AGT expression in transfected 293T cell line by AGT monoclonal antibody (M04), clone 3C10.Lane 1: AGT transfected lysate (Predicted MW: 53.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to AGT on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol