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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10691 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10691, RRID:AB_11189513
- Product name
- HSPD1 monoclonal antibody, clone 3G8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant HSPD1.
- Isotype
- IgG
- Antibody clone number
- 3G8
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) against T-47D (1) , HeLa (2) , HepG2 (3) , A-549 (4) , Jurkat (5) , HEK293 (6) , NIH/3T3 (7) , PC-12 (8) and COS-7 (9) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) .
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (A) , kidney cancer tissues (B) , breast cancer tissues (C) and colon cancer tissues (D) using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using HSPD1 monoclonal antibody, clone 3G8 (Cat # MAB10691) (green) and negative control (purple) .
- Validation comment
- Flow Cytometry
