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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 200-401-F62 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-F62, RRID:AB_2612172
- Product name
- Anti-HSP60 (RABBIT) Antibody - 200-401-F62
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Effects of vitamin D (VD3) supplementation on the brain mitochondrial function of male rats, in the 6-OHDA-induced model of Parkinson's disease.
Araújo de Lima L, Oliveira Cunha PL, Felicio Calou IB, Tavares Neves KR, Facundo HT, Socorro de Barros Viana G
Neurochemistry international 2022 Mar;154:105280
Neurochemistry international 2022 Mar;154:105280
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of rabbit anti-Hsp60 antibody. Lane 1: 100ng Hsp60 recombinant protein. Lane 2: 7.5µg SKBR3 human lysate. Lane 3: 7.5µg MDCK dog lysate. Lane 4: 7.5µg MEF mouse lysate. Primary antibody: Hsp60 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61.1 kDa for Hsp60. Other band(s): none.
- Validation comment
- Western Blot
