ADI-SPA-806-F

antibody from Enzo Life Sciences

Targeting: HSPD1 GroEL, HSP60, SPG13

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ADI-SPA-806-F

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-SPA-806-F, RRID:AB_11177888

Product name

HSP60 monoclonal antibody (LK-1)

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Protein G affinity purified.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus

Host

Mouse

Isotype

IgG

Antibody clone number

LK-1

Vial size

200 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Cross-omics comparison of stress responses in mesothelial cells exposed to heat- versus filter-sterilized peritoneal dialysis fluids.

Kratochwill K, Bender TO, Lichtenauer AM, Herzog R, Tarantino S, Bialas K, Jörres A, Aufricht C
BioMed research international 2015;2015:628158

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Cytotoxic Effects of Tropodithietic Acid on Mammalian Clonal Cell Lines of Neuronal and Glial Origin.

Wichmann H, Vocke F, Brinkhoff T, Simon M, Richter-Landsberg C
Marine drugs 2015 Nov 27;13(12):7113-23

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Molecular characterization and expression analysis of hsp60 gene homologue of sheep blowfly, Lucilia cuprina.

Kumar Singh M, Janardhan Reddy PV, Sreedhar AS, Tiwari PK
Journal of thermal biology 2015 Aug;52:24-37

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Postexercise whole body heat stress additively enhances endurance training-induced mitochondrial adaptations in mouse skeletal muscle.

Tamura Y, Matsunaga Y, Masuda H, Takahashi Y, Takahashi Y, Terada S, Hoshino D, Hatta H
American journal of physiology. Regulatory, integrative and comparative physiology 2014 Oct 1;307(7):R931-43

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Identification and characterization of the ER/lipid droplet-targeting sequence in 17beta-hydroxysteroid dehydrogenase type 11.

Horiguchi Y, Araki M, Motojima K
Archives of biochemistry and biophysics 2008 Nov 15;479(2):121-30

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Elevated heat shock protein 60 levels are associated with higher risk of coronary heart disease in Chinese.

Zhang X, He M, Cheng L, Chen Y, Zhou L, Zeng H, Pockley AG, Hu FB, Wu T
Circulation 2008 Dec 16;118(25):2687-93

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Human MUC1 carcinoma-associated protein confers resistance to genotoxic anticancer agents.

Ren J, Agata N, Chen D, Li Y, Yu WH, Huang L, Raina D, Chen W, Kharbanda S, Kufe D
Cancer cell 2004 Feb;5(2):163-75

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western Blot Analysis of Hsp60 mAb (LK-1) (Prod. No. ADI-SPA-806): Lane 1: MW Marker, Lane 2: Hsp60 (human) (recombinant) Protein (Prod. No. ADI-NSP-540), Lane 3: HeLa Cell Lysate Heat Shocked (Prod. No. ADI-LYC-HL101), Lane 4: PC-12 Cell Lysate Heat Shocked (Prod. No. ADI-LYC-PC101), Lane 5: 3T3 Cell Lysate Heat Shocked (Prod. No. ADI-LYC-3T101)

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of human adrenal tissue stained with HSP60, mAb (LK-1) at 10µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or HSP60 antibody (LK-1) (right).