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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AM03145PU-L - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM03145PU-L, RRID:AB_1617327
- Product name
- anti HSP60
- Antibody type
- Monoclonal
- Antigen
- Human HSP60 produced through recombinant DNA methods in E.coli.
- Reactivity
- Human, Mouse, Rat, Bacteria, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Simian, Yeast
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LK2
- Vial size
- 0.2 mg
- Concentration
- 1.0 mg/ml
Submitted references Interaction of antibodies against cytomegalovirus with heat-shock protein 60 in pathogenesis of atherosclerosis.
Experimental autoimmune encephalomyelitis. Qualitative and semiquantitative differences in heat shock protein 60 expression in the central nervous system.
Bason C, Corrocher R, Lunardi C, Puccetti P, Olivieri O, Girelli D, Navone R, Beri R, Millo E, Margonato A, Martinelli N, Puccetti A
Lancet 2003 Dec 13;362(9400):1971-7
Lancet 2003 Dec 13;362(9400):1971-7
Experimental autoimmune encephalomyelitis. Qualitative and semiquantitative differences in heat shock protein 60 expression in the central nervous system.
Gao YL, Brosnan CF, Raine CS
Journal of immunology (Baltimore, Md. : 1950) 1995 Apr 1;154(7):3548-56
Journal of immunology (Baltimore, Md. : 1950) 1995 Apr 1;154(7):3548-56
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
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- Experimental details
- Gels were loaded with 1, 2.5, 5.0 and 7.5 µg/lane of HeLa and Raw204.4 cell lysates. 0.2 µg/ml primary antibody was used, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti Mouse IgG (H+L) secondary antibody at 1/20.000 dilution was used.
