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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319477 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-ASPP1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-ASPP1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PPP1R13B
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.33 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
- Validation comment
- WB
