NB110-40684

antibody from Novus Biologicals

Targeting: PPP1R13B ASPP1, KIAA0771, p53BP2-like, p85

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-40684

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-40684, RRID:AB_2237454

Product name

Rabbit Polyclonal ASPP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ASPP1 Antibody [NB110-40684] - Detection of Human ASPP1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ASPP1 Antibody [NB110-40684] - Detection of Human ASPP1 on HeLa whole cell lysates using NB110-40684. ASPP1 was also immunoprecipitated by another rabbit anti-ASPP1 antibody and NB110-40683.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ASPP1 Antibody [NB110-40684] - Detection of human ASPP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for IP at 6 ug per reaction. ASPP1 was also immunoprecipitated by rabbit anti-ASPP1 antibody NB110-40683. For blotting immunoprecipitated ASPP1, NB110-40684 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.