66094-1-IG
antibody from Invitrogen Antibodies
Targeting: AHSG
A2HS, FETUA, HSGA
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 66094-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fetuin-A Monoclonal Antibody (1F6B9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MKSLVLLLC LAQLWGCHSA PHGPGLIYRQ PNCDDPETEE AALVAIDYIN QNLPWGYKHT LNQIDEVKVW PQQPSGELFE IEIDTLETTC HVLDPTPVAR CSVRQLKEHA VEGDCDFQLL KLDGKFSVVY AKCDSSPDSA EDVRKVCQDC PLLAPLNDTR VVHAAKAALA AFNAQNNGSN FQLEEISRAQ LVPLPPSTYV EFTVSGTDCV AKEATEAAKC NLLAEKQYGF CKATLSEKLG GAEVAVTCTV FQTQPVTSQP QPEGANEAVP TPVVDPDAPP SPPLGAPGLP PAGSPPDSHV LLAAPPGHQL HRAHYDLRHT FMGVVSLGSP SGEVSHPRKT RTVVQPSVGA AAGPVVPPCP GRIRHFKV (1-367 aa encoded by BC048198)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F6B9
- Vial size
- 150 µL
- Concentration
- 0.96 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human blood tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-IG (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using AHSG antibody 66094-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-mouse IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 66094-1-Ig (Fetuin-A antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 66094-1-Ig (Fetuin-A antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 66094-1-IG (Fetuin-A antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 66094-1-IG (Fetuin-A antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.20ug Fetuin-A antibody (66094-1-IG, red) and control antibody (blue). Fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Fetuin-A (IP:66094-1-IG, 3ug; Detection:66094-1-IG 1:3000) with HepG2 cells lysate 6000ug.