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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF675 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IGFBP-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IGFBP-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross reactivity with recombinant mouse IGFBP-3 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, and rhIGFBP-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Netazepide Inhibits Expression of Pappalysin 2 in Type 1 Gastric Neuroendocrine Tumors.
ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model.
Amyloid beta-mediated epigenetic alteration of insulin-like growth factor binding protein 3 controls cell survival in Alzheimer's disease.
Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3.
The contributions of HIF-target genes to tumor growth in RCC.
The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C.
Human IGF binding protein-3 overexpression impairs glucose regulation in mice via an inhibition of insulin secretion.
Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
IGFBP-3 is a metastasis suppression gene in prostate cancer.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Lloyd KA, Parsons BN, Burkitt MD, Moore AR, Papoutsopoulou S, Boyce M, Duckworth CA, Exarchou K, Howes N, Rainbow L, Fang Y, Oxvig C, Dodd S, Varro A, Hall N, Pritchard DM
Cellular and molecular gastroenterology and hepatology 2020;10(1):113-132
Cellular and molecular gastroenterology and hepatology 2020;10(1):113-132
ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model.
Xiang Q, Liao Y, Chao H, Huang W, Liu J, Chen H, Hong D, Zou Z, Xiang AP, Li W
Stem cell research & therapy 2018 Feb 26;9(1):51
Stem cell research & therapy 2018 Feb 26;9(1):51
Amyloid beta-mediated epigenetic alteration of insulin-like growth factor binding protein 3 controls cell survival in Alzheimer's disease.
Sung HY, Choi EN, Lyu D, Mook-Jung I, Ahn JH
PloS one 2014;9(6):e99047
PloS one 2014;9(6):e99047
Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3.
Musil J, Kutinova L, Zurkova K, Hainz P, Babiarova K, Krystofova J, Nemeckova S
Cancer gene therapy 2014 Mar;21(3):115-25
Cancer gene therapy 2014 Mar;21(3):115-25
The contributions of HIF-target genes to tumor growth in RCC.
Zhang T, Niu X, Liao L, Cho EA, Yang H
PloS one 2013;8(11):e80544
PloS one 2013;8(11):e80544
The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C.
Niu X, Zhang T, Liao L, Zhou L, Lindner DJ, Zhou M, Rini B, Yan Q, Yang H
Oncogene 2012 Feb 9;31(6):776-86
Oncogene 2012 Feb 9;31(6):776-86
Human IGF binding protein-3 overexpression impairs glucose regulation in mice via an inhibition of insulin secretion.
Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
Endocrinology 2011 Jun;152(6):2184-96
Endocrinology 2011 Jun;152(6):2184-96
Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
Tyan SW, Kuo WH, Huang CK, Pan CC, Shew JY, Chang KJ, Lee EY, Lee WH
PloS one 2011 Jan 13;6(1):e15313
PloS one 2011 Jan 13;6(1):e15313
IGFBP-3 is a metastasis suppression gene in prostate cancer.
Mehta HH, Gao Q, Galet C, Paharkova V, Wan J, Said J, Sohn JJ, Lawson G, Cohen P, Cobb LJ, Lee KW
Cancer research 2011 Aug 1;71(15):5154-63
Cancer research 2011 Aug 1;71(15):5154-63
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IGFBP-3 in Human Colon. IGFBP-3 was detected in immersion fixed paraffin-embedded sections of human colon using 10 µg/mL Human IGFBP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IGFBP-3 Inhibition of IGF-II-dependent Cell Proliferation and Neutralization by Human IGFBP-3 Antibody. Recombinant Human IGFBP-3 (Catalog # 675-B3) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-3 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF675). The ND50 is typically 0.75-3.0 µg/mL.
