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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB305-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGFBP-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IGFBP-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant human (rh) IGF-I R, rhIGFBP-1, rhIGFBP-2, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA46087- Isotype
- IgG
- Antibody clone number
- 84728
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The participation of insulin-like growth factor-binding protein 3 released by astrocytes in the pathology of Alzheimer's disease.
IGFBP3 promotes esophageal cancer growth by suppressing oxidative stress in hypoxic tumor microenvironment.
Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
Human IGF binding protein-3 overexpression impairs glucose regulation in mice via an inhibition of insulin secretion.
Epidermal growth factor receptor regulates aberrant expression of insulin-like growth factor-binding protein 3.
Watanabe K, Uemura K, Asada M, Maesako M, Akiyama H, Shimohama S, Takahashi R, Kinoshita A
Molecular brain 2015 Dec 4;8(1):82
Molecular brain 2015 Dec 4;8(1):82
IGFBP3 promotes esophageal cancer growth by suppressing oxidative stress in hypoxic tumor microenvironment.
Natsuizaka M, Kinugasa H, Kagawa S, Whelan KA, Naganuma S, Subramanian H, Chang S, Nakagawa KJ, Rustgi NL, Kita Y, Natsugoe S, Basu D, Gimotty PA, Klein-Szanto AJ, Diehl JA, Nakagawa H
American journal of cancer research 2014;4(1):29-41
American journal of cancer research 2014;4(1):29-41
Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
Elzi DJ, Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):12052-7
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 24;109(30):12052-7
Human IGF binding protein-3 overexpression impairs glucose regulation in mice via an inhibition of insulin secretion.
Nguyen KH, Yao XH, Moulik S, Mishra S, Nyomba BL
Endocrinology 2011 Jun;152(6):2184-96
Endocrinology 2011 Jun;152(6):2184-96
Epidermal growth factor receptor regulates aberrant expression of insulin-like growth factor-binding protein 3.
Takaoka M, Harada H, Andl CD, Oyama K, Naomoto Y, Dempsey KL, Klein-Szanto AJ, El-Deiry WS, Grimberg A, Nakagawa H
Cancer research 2004 Nov 1;64(21):7711-23
Cancer research 2004 Nov 1;64(21):7711-23
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- IGFBP-3 in Human Placenta. IGFBP-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human IGFBP-3 Monoclonal Antibody (Catalog # MAB305) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
