679702

antibody from BioLegend
Targeting: IGFBP3 BP-53, IBP3
Provider product page for 679702

Antibody data

Product number
679702 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#679702, RRID:AB_2566243
Product name
Purified anti-IGFBP-3
Antibody type
Monoclonal
Antigen
Human IGFBP-3, amino acids (Gly28-Glu291) (Accession# X64875) with an N-terminal Met, was expressed in 293E cells.
Description
The insulin-like growth factor system includes two receptors (IGF-1R and IGF-2R), two ligands (IGF-1 and IGF-2), and seven binding proteins (IGFBP1-7). The binding proteins are produced in the liver and bind to the IGFs with different affinities, thus regulating the interaction of the IGFs with their receptors. Insulin-like growth factor binding protein-3 (IGFBP-3) is the major serum carrier protein for IGF proteins. IGFBP-3 sequesters IGF in a stable ternary complex with the acid-labile subunit (ALS). IGFBP-3 binds IGF-1 with high affinity and specificity and modulates IGF-1 action. IGFBP-3 undergoes limited enzymatic proteolysis to generate small N-terminal fragments. These proteolytic products lose their affinity for IGF-1 and IGF-2. Nevertheless, they preserve IGF independent activity. In that sense, a 16 kD fragment (1-95-IGFBP-3) and the full length molecule can potentiate apoptosis in colorectal epithelial cells, and this apoptotic process is associated with the inhibition of TNF-α-induced NF-κB activity. In addition, IGFBP-3 has been detected in the cell nucleus and interacts with 9-cis retinoic acid receptor-alpha (RXR-α), which is a nuclear receptor that is required for the apoptosis-inducing effects of IGFBP-3. IGFBP-3R physically interacts and activates caspase-8 and mediates IGFBP-3-induced apoptosis and tumor suppression.
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Antibody clone number
M6501G03
Vial size
100 µg
Concentration
0.5 mg/ml
Storage
2°C-8°C
Handling
Ambient RT
IGFBP3 protein structure - 679702 shown in red.